NRK1 controls nicotinamide mononucleotide and nicotinamide riboside metabolism in mammalian cells

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NRK1 controls nicotinamide mononucleotide and nicotinamide riboside metabolism in mammalian cells

NAD+ is a vital redox cofactor and a substrate required for activity of various enzyme families, including sirtuins and poly(ADP-ribose) polymerases. Supplementation with NAD+ precursors, such as nicotinamide mononucleotide (NMN) or nicotinamide riboside (NR), protects against metabolic disease, neurodegenerative disorders and age-related physiological decline in mammals. Here we show that nico...

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Nicotinamide riboside kinases display redundancy in mediating nicotinamide mononucleotide and nicotinamide riboside metabolism in skeletal muscle cells

OBJECTIVE Augmenting nicotinamide adenine dinucleotide (NAD+) availability may protect skeletal muscle from age-related metabolic decline. Dietary supplementation of NAD+ precursors nicotinamide mononucleotide (NMN) and nicotinamide riboside (NR) appear efficacious in elevating muscle NAD+. Here we sought to identify the pathways skeletal muscle cells utilize to synthesize NAD+ from NMN and NR ...

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Identification of Isn1 and Sdt1 as Glucose- and Vitamin-regulated Nicotinamide Mononucleotide and Nicotinic Acid Mononucleotide 5 -Nucleotidases Responsible for Production of Nicotinamide Riboside and Nicotinic Acid Riboside*

Katrina L. Bogan, Charles Evans , Peter Belenky, Peng Song, Charles F. Burant , Robert Kennedy, and Charles Brenner From the Biochemistry Graduate Program, Dartmouth Medical School, Lebanon, New Hampshire 03756, the Department of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, and the Department of Chemistry and the Michigan Metabolomics and Obesity Center, ...

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Nicotinamide Riboside Promotes Sir2 Silencing and Extends Lifespan via Nrk and Urh1/Pnp1/Meu1 Pathways to NAD+

Although NAD(+) biosynthesis is required for Sir2 functions and replicative lifespan in yeast, alterations in NAD(+) precursors have been reported to accelerate aging but not to extend lifespan. In eukaryotes, nicotinamide riboside is a newly discovered NAD(+) precursor that is converted to nicotinamide mononucleotide by specific nicotinamide riboside kinases, Nrk1 and Nrk2. In this study, we d...

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Nicotinamide mononucleotide pyrophosphorylase activity in animal tissues.

An enzyme which catalyzes the formation of nicotinamide mononucleotide in the presence of nicotinamide, S-phosphoribosylpyrophosphate, adenosine triphosphate, and Mgff has been purified from rat liver. The reaction is highly specific for ATP and is not substituted for by a variety of nucleotides. Enzymic activity has been found in all of the tissues studied, and in the crude homogenate it is ap...

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ژورنال

عنوان ژورنال: Nature Communications

سال: 2016

ISSN: 2041-1723

DOI: 10.1038/ncomms13103